Conventional kinesins are molecular motors that walk along microtubules to carry out a large variety of cellular transport processes. The conventional kinesin of the filamentous fungus Neurospora crassa is 3-4 times faster than its animal relatives. Its neck domain displays characteristic properties that is also reflected in a specific sequence pattern. In the present work the functional roles of the specific neck and hinge domains for mechano-chemistry, dimerization and regulation of fast fungal kinesins are investigated. To address this issue, mutant protein constructs were generated, and...