Xyloglucans and rhamnogalacturonan II (RG-II) are fucosylated cell wall compounds and play crucial roles in plant development. One aim of this work was to better understand xyloglucan modification or degradation, and more specifically to get information on enzyme(s) responsible for the removal of L-fucose residue present on this polysaccharide. Two candidates were investigated, AtFUC95A and AtFXG1, which have been described as xyloglucan-specific 1,2-fucosidases, even though they are phylogenetically not related. Both were investigated in Arabidopsis knockout mutants as well as by transient...