The potential of cold-active proteases are greater than thermostable proteases in view of their high catalytic efficiency at low and moderate temperatures. However, these enzymes along with their producing organisms are remains unexplored. The aim of present work was to isolate, purify and characterize novel cold-active extracellular proteases and explore its biotechnological potential. As a result, two potential cold-adapted bacteria viz. C. luteum and S. maltophilia are isolated that producing cold-active metallo-proteases and alkaline protease, respectively. The enzyme from S. maltophilia...