This detailed volume presents a series of protocols dealing with different aspects of inclusion body (IB) processing, from cloning procedures to purification of refolded product. Commencing with chapters on upstream processing, looking into different expression strategies for IB production, the book continues with downstream applications, highlighting early protein purification and subsequent analytics, as well as success stories of IB-based processes. Written for the highly successfulMethods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the...
This detailed volume presents a series of protocols dealing with different aspects of inclusion body (IB) processing, from cloning procedures to purif...