This thesis presents research on novel laboratory-scale synchrotron X-ray sources based on inverse Compton scattering and applications of their X-ray radiation using the Munich Compact Light Source (MuCLS) as an example. It provides an introduction to the theory of this laser-electron interaction, laser resonators and X-ray interactions with matter. On this basis, upgrades to the laser system including the development of a new laser optic, X-ray beam stabilisation and two techniques for fast X-ray energy switching of inverse Compton sources are presented. On the application side, the...