The proceedings of MG16 give a broad view of all aspects of gravitational physics and astrophysics, from mathematical issues to recent observations and experiments. The scientific program of the meeting included 46 plenary presentations, 3 public lectures, 5 round tables and 81 parallel sessions arranged during the intense six-day online meeting. All talks were recorded and are available on the ICRANet YouTube channel at the following link: www.icranet.org/video_mg16.These proceedings are a representative sample of the very many contributions made at the meeting. They contain 383 papers,...