The present investigation is envisaged to study the production of alkaline and thermostable polygalacturonase using isolated soil Bacillus subtilis(C4) by submerged fermentation. A total of 66 bacterial colonies were isolated from six sampling sites. Out of 66 isolates 8 isolates showed polygalacturonase activity and C4 isolate showed maximum activity. The new isolate polygalacturonase potential was tested against three polygalacturonase producing microorganism. Comparatively isolate C4 showed maximum enzyme activity hence it was chosen for in depth study. The isolate C4 was characterized and...