Recently, in the field of tissue engineering, fabrication of three dimensional (3D) electrospun membrane is much emphasized. 3D scaffold fabricated from multi-layered electrospun membrane possess both the benefits of nanofibrous and microfibrous scale morphology in one scaffold. In this book, layered composite scaffolds or membranes were fabricated using blend of Polycaprolactone (PCL) /Chitosan and PCL layer-by-layer using multilayer electrospinning techniique. Characterization of membranes were conducted using several techniques. Using optimized solution concentration and processing...