This research was carried out to determine sand fly vector(s), infection of Phlebotomus orientalis with Leishmania parasites and possible animal reservoir of visceral leishmaniasis in villages and Dinder National Park (DNP) in eastern Sudan. Samples of P. orientalis females were collected from villages in Gedarif State and DNP. The collected females were dissected for present of Leishmania promastigotes as well as analysed by PCR for DNA of the parasites. Blood samples from small wild mammals and domestic dogs were smeared on glass slides and blotted on filter papers Whatmann # 3 for...

This research was carried out to study the morphometric and molecular differences in populations of Phlebotomus orientalis from three different regions in Sudan. Samples of Phlebotomine sandflies were collected from Gedaref, White Nile and Khartoum States. The specimens of P. orientalis were collected using CDC miniature light traps and sticky paper traps. The sand fly specimens were then mounted on glass slide on Breleses medium and identified to species level. Then mounted samples of P. orientalis were processed for morphometric measurements of 23 and 29 characters for females and males...

This study was carried out as entomological surveys at White Nile and Khartoum States during April-July 2011. The study was conducted to investigate the fauna, abundance and distribution of sand flies in the two areas. Sand flies were collected using CDC miniature light traps and sticky paper traps. In addition, female of Phlebotomus sand flies were dissected for Leishmania parasite and to determine parous rates in the White Nile state. Fourteen species of sand flies were recorded: six Phlebotomus sand flies and eight Sergentomyia species. No Leishmania parasites were detected in sand flies...

This study was conducted to investigate the swarming and mating behaviors of wild and irradiated males An. Arabiensis. Male mosquitoes were irradiated as pupae and transported as adults to the field. The wild ones were collected as larvae in the field and reared to adult. Both irradiated and wild-reared insects were dusted with fluorescent powder (PF). Search for swarming insects started before the sunset. When allocated, males and coupling pairs were sampled from swarms. Captured females were dissected under microscope for parity and insemination rates. Wings of males from swarms and in...

This study was carried out elucidate genetic variation among populations of Phlebotomus orienanlis vector of visceral leishmnaiasis from three different geographical regions in Sudan. Sand flies were collected from White Nile, Gedarif, Blue Nile and Khartoum States using CDC and sticky paper traps. Specimens collected from these areas were analysed using Rapid Amplified Polymorphic DNA -Polymerase Chain Reaction(RAPD-PCR). RAPD-PCR was performed using 30 random primers. Population of each site investigated showed a characteristic diagnostic DNA bands. Furthermore, of P. orientalis showed...

This study was carried out to assess the impact of long-term colonisation, irradiation, and transportation on male Anopheles arabiensis vigour and mating competitiveness and the dispersal of irradiated ones in the field. Resting behavior and survival of males, and productivity of larval habitats were also investigated. Male insects were irradiated as pupae and transported with colonised ones to the filed site. Wild males and females were collected as larvae and reared to adult. Colonised or irradiated males were released in a contained semi-field system with wild conspecifics and virgin...

This research was carried out to study for the first time the colonization, the biology and to collect first baseline data on susceptibility status of Phlebotomus papatasi the vector of Cutaneous Leishmaniasis (CL) in Sudan. Samples of P. papatasi females were collected from Surogia village (located at the north site of Khartoum State, Sr), White Nile forest (White Nile State, WN) and Dinder National Park located at southern east of Sudan (Gedaref State, DNP). The P. papatasi females were collected with different methods using both light and sticky oil traps. A colony of P. papatasi was...