This hand book details the process of Cataractogenesis using 'Lens Organ Culture Technique' and the changes in the lens enzymes. Three models were selected viz sugar induced cataract, hydrogen Peroxide induced cataract and steroid induced cataract. A total of 480 Goat lenses, obtained from slaughter house were analyzed. These lenses were subjected to 'Lens Organ Culture Technique'. The cultured lenses were categorized as control group and the experimental groups (Sugar induced cataract H202 induced cataract and steroid induced cataract). It was found that total soluble proteins were...

Benign thyroid nodules are common in adults and thyroid malignancy is the most common malignancy of endocrine system. Papillary thyroid carcinoma and follicular thyroid carcinoma are often curable and prognosis is good if identified in early stage.To avoid unnecessary surgery 'Fine needle aspiration cytology' (FNAC) is used as a diagnostic tool to differentiate benign nodule from malignant nodule. To improve the diagnostic accuracy of FNAC, silver nitrate staining technique is applied. Smear from patients is stained with silver nitrate and nucleolar organising regions (segments of DNA) were...

This book emphasizes the importance of levels of lactate dehydrogenase (LDH) isoenzyme in ascitic fluid as an aid to differential diagnosis, of portal hypertension (P.H.T.). Total LDH activity and LDH isoenzymes levels in ascitic fluid and in serum of patients from ascites not associated with P.T.H. and ascites associated with P.T.H are presented. Concentration of total protein and protein fractions are also included to correlate with LDH total activity and LDH isoenzymes level. Increase in levels of LDH total activity in ascitic fluid and in serum in patients of ascites associated with...