This book is based on two stage biogas production from waste textiles. The global consumption of textile is increasing and the end life results as a waste. Such cotton based waste textiles could be converted into energy and here we have showcased the possibility to convert it. We have used four different materials such as jeans, pretreated jeans, cotton and starch to compare. The results showed that pretreated jeans had a higher biogas production compared to untreated jeans.