The presence of Helicobacter pylori in sewage water was confirmed, thus there is a reservoir for H. pylori outside of the human stomach. In aquatic environment, this bacterium transforms from the characteristic bacillary form to coccal or cocco-bacilli form, which is non culturable. However, Culturing this bacterium for the first time by the use of MDCS method took place. Here is a similarity between H. pylori found in sewage and those obtained from clinical biopsy and drinking water as indicated by RFLP technique raising the possibility for a fecal-oral route of transmission