Nigella sativa is an annual flowering plant, found in south and southwest Asia. Nigella sativa oil contains conjugated linoleic acid, thymoquinone, nigellone (dithymoquinone), melanthin, nigilline, damascenine, and tannins. In present study, a comparison was made among the extraction efficacy of cold maceration, soxhlet extraction and microwave assisted extraction. Cold maceration of black seeds gave maximum extract after 21 hours of extraction. A maximum of 6.75 g of extract was obtained by soxhlet extraction of Nigella sativa. While Microwaves produced maximum extract (15 g/gram of plant...