Present study was designed to investigate the clinico-pathological changes produced in broilers naturally infected with Newcastle Disease (ND) and to standardize the RT-PCR technique for confirmatory diagnosis of ND. A field survey of commercial broiler poultry farms (n=50) suspected of being infected with ND was carried out in district Faisalabad, Pakistan. The suspected birds either live or fresh mortality from each suspected infected premises were collected and pooled tissue samples were used for histopathological study, virus isolation identification and RT-PCR. Out of total 50 samples...