In this book, we demonstrate a new single cell optoporation and transfection technique using femtosecond Gaussian laser beam and optical tweezers. Tightly focused near-infrared (NIR) femtosecond laser pulse was employed to transiently perforate the cellular membrane at a single point of MCF-7 cancer cell. A distinct technique is developed by trapping the microparticle using optical tweezers to focus femtosecond laser exactly on the cell membrane to puncture it for the desired time. Subsequently, calculated amount of external gene was introduced in the cell by trapping and inserting same...
In this book, we demonstrate a new single cell optoporation and transfection technique using femtosecond Gaussian laser beam and optical tweezers. Tig...
