Actinomycetes are aerobic gram positive filamentous bacteria with high G+C (Guanine + Cytosine) content which form asexual spores and which are widely distributed in both terrestrial and aquatic habitats. They are the main source of clinically important antibiotics, most of which are too complex to be synthesized by combinatorial chemistry. L-glutaminase is an enzyme produced by various microorganisms which is used for the treatment of leukemia, as flavour enhancer and as enzyme sensor. Even though L-glutaminase activity was reported in various microorganisms, L-glutaminase from actinomycetes...

Solid-state fermentation was carried out for the production of extra-cellular L-methioninase by Aspergillus flavipes MTCC 6337 using ten agro-industrial residues. Sesame oil cake was selected as solid substrate for L-methioninase production by A. flavipes. The maximum L-methioninase yield (396 U/gds) was obtained in preliminary studies by incubating flasks containing sesame oil cake of 60% initial moisture content (pH 8.0) as substrate supplemented with D-glucose (1.0% w/v), L-methionine (0.8% w/v), MnSO4 (0.5% w/v) and 3 ml of A. flavipes conidial suspension for 8 days at 30 0C. 4 parameters...