Actinomycetes are aerobic gram positive filamentous bacteria with high G+C (Guanine + Cytosine) content which form asexual spores and which are widely distributed in both terrestrial and aquatic habitats. They are the main source of clinically important antibiotics, most of which are too complex to be synthesized by combinatorial chemistry. L-glutaminase is an enzyme produced by various microorganisms which is used for the treatment of leukemia, as flavour enhancer and as enzyme sensor. Even though L-glutaminase activity was reported in various microorganisms, L-glutaminase from actinomycetes...