A fifteen different biphasic media were used to cultivated promastigote parasite of Leishmania donovani and compared with ordinary NNN medium, to find a cow milk agar medium can provide a good cultivation to the parasite as well as the ordinary medium, where milk is a simple, cheap and a good source of protein, carbohydrate, lipids and calcium. But milk cannot be a substituted for rabbit blood as solid phase that was given the greatest count of parasites when using it. Also, using a 10% of human urine as a liquid phase can enhance the growth rate of parasite up to eight times, this reduced...