The aim of this manual is to provide a comprehensive guide to the methods involved in collecting, preparing and screening plants for bioactive properties for manipulating key ruminal fermentation pathways and against gastrointestinal pathogens. The manual will better equip the reader with methodological approaches to initiate screening programmes to test for bioactivity in native plants and find natural alternatives to chemicals for manipulating ruminal fermentation and gut health. The manual provides isotopic and non-isotopic techniques to efficiently screen plants or plant parts for a...