A stand-alone laboratory manual for molecular biophysics, this book presents essential techniques that need to be understood by any experimentalist working with biological samples. The organization of topics follows a logical experimental progression, beginning with a DNA construct, which is amplified, mutated, or fused to another gene, expressed in bacteria, and purified as either a protein or plasmid DNA. Readers are shown how the purified protein is characterized using spectroscopy, crystallography, and lipid bilayer expression. Finally, they see how the purified plasmid DNA is...
A stand-alone laboratory manual for molecular biophysics, this book presents essential techniques that need to be understood by any experimentalist...
"essential reading for any physical scientist who is interested in performing biological research." ―Contemporary Physics
"an ambitious text.... Each chapter contains protocols and the conceptual reasoning behind them, which is often useful to physicists performing biological experiments for the first time." -Physics Today
This fully updated and expanded text is the best starting point for any student or researcher in the physical sciences to gain firm grounding in the techniques employed in molecular biophysics and...
Praise for the First Edition
"essential reading for any physical scientist who is interested in performing biological research." ―...
