Metabolic engineering targets for the production of high-value compounds. Citrus peel is produced 15,000,000 t per year worldwide causing environmental problems. Its main constituent is D-galacturonic acid and can be converted to useful chemicals using genetically engineered strains. Engineered Aspergillus niger were used in solid and submerged state fermentation to convert orange peel to L-galactonate in a consolidate process. The gaaB coding for L-galactonate dehydrogenase was deleted ( gaaB) and ii) the gaaB was deleted and the gaaA coding for D-galacturonate reductase was overexpressed (...
Metabolic engineering targets for the production of high-value compounds. Citrus peel is produced 15,000,000 t per year worldwide causing environmenta...