In conventional fed batch process development approaches, batch operating parameters are kept constant and only feeding parameters such as feeding time, post-feed concentration are manipulated, whereas any interactions among the batch and fed-batch operating parameters are not evaluated. In this study an attempt was made to study the interaction between fed-batch operating parameter-post feed glucose concentration (A) and batch operating parameters- seeding density (B), temperature (C), and dissolved oxygen concentration (D) by their simultaneous manipulation, as well as the effect of these...