Tomato is one of the most important vegetable crops throughout the world and is available in almost all seasons of the year in tropical and sub-tropical regions. Various conditions for the in vitro germination of tomato were optimized. When seeds were inoculated in half strength MS medium supplemented with 2mg/L BAP and 2mg/L of NAA reduced number of days was recorded for seed germination. A vector having reporter gene GUS was transformed into the four varieties of tomato cultivars. ViaAgrobacterium mediated transformation method. For all four cultivars two types of explants were used...