In a case where phosphate content of cane juice is deficient, it is necessary to compensate the deficiency by external addition of phosphoric acid. To settle the precipitate formed, optimum amount of flocculant needs to be used. Therefore, this study was conducted to determine the optimum conditions of phosphate (P2O5) and separan. Two cane varieties, B52298 and NCo 334 were studied in terms of turbidity, settling rate, mud volume. The experiment was conducted using Central Composite Design (CCD). The optimum levels for the B52298 cane variety were 228.7 mg/L P2O5 and 3.35 ppm separan, which...