The present work helped to confirm the disease after feeding of the contaminated groundnut hay. The fungi isolated were Rhizopus oryzae, Fusarium sporotrichoides and Fusarium semitectum. Macroscopic, microscopic and molecular characterization of the fungi were done for confirmation. The fungi contaminated wheat materials were subjected to mycotoxins analysis using LC-MS/MS method. Fusarium sporotrichoides infected material had had apicidin 28.80 ug/kg and beauvericin 5126.67 ug/kg.Fusarium semitectum infected material was having apicidin 5375 ug/kg and beauvericin 2732.50 ug/kg of infected...