Nuclear Magnetic Resonance (NMR) spectroscopy has made a tremendous impact in many areas of chemistry, biology and medicine. High resolution NMR spectroscopy has been established as a powerful non-destructive and non-invasive technique with high specificity for measuring low-molecular weight metabolites in the body fluids and tissue extract that can provide complete structural analysis of a wide range of organic molecules in various pathological conditions. So, in this regard, NMR based metabonomics approach is presented here for the development of the extraction methods as well as possible...