Four locally grown varieties of tomato; BINA-3, BARI-3, Bahar, Maple and one Indian variety; Pussa Rubby (PR) were selected in this study to observe the effects of growth regulators(in different concentrations&combinations)on shoot initiation and root induction. MS media containing 1.5mg/l BAP and 0.2mg/l IAA showed best result with lowest callus formation and increased number of shoot in all the varieties.In this medium the best response was found for Maple(93.33%)followed by Pussa Ruby(86.67%)and for the other varieties such as BARI-3, BINA-3& Bahar gave almost similar response to that...