The use of human primary cells provide researchers in different areas with irrefutable more biologically relevant data than using cell lines or animal blood cells. This work was performed in the scope of the Cell Biology Services @ CEDOC, a platform aiming to provide viable and trustful human primary cells and products. We had three main objectives: protocol optimizations for human blood cell isolation, culture and cryopreservation; cost estimation and divulgation of the services. In this book, we review standard protocols and compare different strategies for blood cell isolation (peripheral...