HMG-CoA reductase catalyzes the conversion of HMG-CoA to mevalonate, which is known as the rate-limiting step in the biosynthesis of cholesterol and other intermediates of mevalonate pathway.The purified plasmid (pGEXtev/hmgr) containing gene of human HMG-CoA reductase was confirmed by restriction analysis and sequencing. After verification, it was transformed into competent cells of Escherchia coli (BL21 and DH5 ) for which DH5 produced most promising results. Protein expression was induced with IPTG and further optimized through varying the concentration of IPTG, betain, sorbitol and...
HMG-CoA reductase catalyzes the conversion of HMG-CoA to mevalonate, which is known as the rate-limiting step in the biosynthesis of cholesterol and o...
