The worldwide demand of citric acid is about 6x105 tons per year. Citric acid, with an estimated annual production of 500,000 tons, is produced almost exclusively by fermentation with Aspergillus niger and widely used in food, chemical, pharmaceutical and other industries. Keeping in view production of citric acid was investigated in submerged fermentation using shake flask fermentation. Maximum product yield was achieved under optimum methanol concentration (1.5 %) and ethanol concentration (1.0%) which shows enhance improvement in citric acid production compared to the control strain...