Gemifloxacin mesylate is a novel fourth generation fluoroquinolone agent that is used for the treatment of community acquired pneumonia and acute bacterial exacerbation of chronic bronchitis that provide high rates of mortality worldwide. Spectrofluorimetric analysis constitutes a widespread, effective technique to improve analysis selectivity and sensitivity. Moreover, it offers easy and less time-consuming analysis by using simple and available reagents, which are able to be used for routine determinations of drug substances. Therefore, spectrofluorimetric analysis is one of the major...