CHMO originating from Acinetobacter NCIB 9871 was chosen for this study. Mutations to stabilize helical structure motifs in particular were predicted by sequence comparison with the structure of PAMO from Thermobifida. Certain combinatorial and rational protein design approaches were applied for the development of several generations of mutants. Parallel screening methods, both on whole-cells and crude cell extracts, were utilized to assess biocatalytic performance. These mutants were screened for their thermostability according to the protocols established for this study. ThermoFAD...