Ethanolic extract of Xanthium indicum plant was prepared and tested for its phytochemical and pharmacological investigation. Preliminary phytochemical screening showed that extracts possessed the active principles-alkaloid, carbohydrate, glycoside, flavonoid & Tannin. The extract showed antioxidant property(IC50 ==136.45 µg/mL) compare to standard (ascorbic acid, IC50 =12.16 µg/mL). The extract also shows antioxidant activity by reducing FeCl3. The extract inhibited the acetic acid induced writhing in mice (47.5% P<0.001and 24.54% P<0.05at the dose of 500 and 250 mg/kg body weight...