High ethanol accumulation capacity and high ethanol tolerance are crucial in several industrial applications (Kerr, 2010). Identification of causative genes underlying those polygenic traits have being cumbersome to perform and novel methodologies to facilitate the studies of industrial yeast strains are aspired (Mackay et al., 2009). We have screened 68 different yeast strains in very high gravity fermentations and we have identified a diploid sake strain that produces 18-19% (v/v) ethanol. This strain is able to accumulate nearly 21% (v/v) ethanol when continuous stirred fermentation is...