The present study was carried out to spot fairly the limit of tsetse fly belt in river Khor Yabus, from Sudan-Ethiopian border in the Blue Nile State,Sudan as the successive ecological changes might create significant modification in tsetse distribution. Moreover to draw attention to circulating Trypanozoon species via detecting infection on tsetse flies using DNA-PCR technique.The results obtained showed that only 8 flies out of 180 examined (4.44%) harboring Trypanosoma brucei group as they reacted positively with TBR1&2 primers, out of these only 3 flies (37.5%) reacted positively with...