Sendai virus (SeV) is not just a mouse pathogen but is evolving into a cutting-edge component of biotechnology. SeV reverse genetics originating from a pure academic need to settle long-held questions in the biology and pathogenicity of nonsegmented negative strand RNA viruses (Mononegavirales) is about to bear the impressive fruit of multipurpose cytoplasmic (non-integrating) RNA vectors. This book brings together in one source the SeV biology revealed by conventional approaches and reverse genetics, the methods to construct the first-generation SeV vector and to generate safer versions, and...

A wide-ranging review of the SeV vector, a multipurpose cytoplasmic RNA vector with extremely high performance, this volume covers diverse potent applications, including its use as an AIDS vaccine vector and the creation of BioKnife for tumor resection.