Project was planned to study the optimization of production medium, kinetic and characterization of native and entrapped laccase from Pleurotus ostreatus. Production of laccase employing Pleurotus ostreatus during dye decolorization was optimized using different media composition.Purified laccase was immobilized in xerogels using various ratios of PTMOS and TMOS. All immobilized enzyme showed better thermostability and mesostability than native laccase. Although the best method of immobilization might differ from enzyme to enzyme,from application to application and from carrier to...