Project was planned to study the optimization of production medium, kinetic and characterization of native and entrapped laccase from Pleurotus ostreatus. Production of laccase employing Pleurotus ostreatus during dye decolorization was optimized using different media composition.Purified laccase was immobilized in xerogels using various ratios of PTMOS and TMOS. All immobilized enzyme showed better thermostability and mesostability than native laccase. Although the best method of immobilization might differ from enzyme to enzyme,from application to application and from carrier to...

The stipulate for industrial enzymes, predominantly microbial origin is escalating due to their applications in ample diversity of processes. Serine Protease held the prevalent share of the enzyme market and hence microbial proteases have been reckoned comprehensively, with prominence on unusual aspects of proteases.The alkaline serine protease produced form B. subtilis IBL-01 appeared potential source for up-scaling the process and provision at required level to the local detergent and leather industries. Process development in this regard is strongly recommended for uplifting the country̕...