Botryococcus braunii was widely reported to be one of the major bio-resources of hydrocarbon in a variety of oil-rich deposits dating from the Ordovician period to the present. This study aims primarily at isolation of B. braunii from the Egyptian freshwater and to optimize the growth controlling conditions to attain biomass and biofuel production much more feasible and sustainable. Four Egyptian isolates of B. braunii (EG-Bb01, EG-Bb02, EG-Bb03 and EG-Bb04) were isolated and characterized based on growth potentialities, hydrocarbon composition and 5.8s ribosomal RNA genes. Hydrocarbon (%)...