Mesenchymal stem cells (MSC) are a valuable source of multipotent progenitors for regenerative medicine, but may require to be genetically modified to widen their efficacy. For example, overexpression of the angiogenic factor VEGF at controlled levels is an attractive strategy to overcome the crucial bottleneck of graft vascularization and to avoid aberrant vascular growth. Since the regenerative potential of MSC is rapidly lost during in vitro expansion, we developed an optimized technique to achieve high-efficiency retroviral vector transduction of both adipose tissue- and bone...
Mesenchymal stem cells (MSC) are a valuable source of multipotent progenitors for regenerative medicine, but may require to be genetically modified to...