The project is attempt to synthesis of the basic core structure of coelenterazine, found in many acuatic organisoms which is also substrate in many luciferase and photoprotien, aequorin and obelin and to study the Binding interaction with BSA, Brovine Serum Albumine is a serum albumin protein derived from the cows. Also we investigate to know the Binding stoichiometry, site of binding. And the effect of adding surfactant ,SDS (Sodium Dodecyl Sulfate) in presence or without Adding BSA. Also we calculate the quantum yield the synthesized molecule in various solvent and to inform various...