Bovine tuberculosis (BTB) is a chronic infectious disease of animals and humans characterized by the formation of granulomas in tissues and organs, principally caused by Mycobacterium Bovis (M. bovis). A cross-sectional study was conducted from September 2010 to May 2011 with the objectives to determine the prevalence of BTB, to characterize the isolates of Mycobacterium tuberculosis complex, and to assess zoonotic importance of BTB based on questionnaire survey in Setit and Kafta Humera districts, Western Zone of Tigray Region. Thirty one herds comprising 484 cattle were subjected to CIDT...