Trifolium alexandrinum L. (Egyptian clover) is one of the most important forage crops in the world. Its regeneration in tissue culture has been described in few reports but the efficiency, accurate time scales and the applicability to various genotypes of the described procedures are uncertain. Therefore their suitability for genetic transformation is unclear. Egyptian clover (Trifolium alexandrinum L.) is a very important annual nitrogen fixing leguminous crop. It is also an essential component of crop rotation where it must be grown at least once every 2 years to enrich the soil with...