The polymerase chain reaction (PCR) has recently been investigated for detecting Mycobacterium tuberculosis in clinical specimens and appears to have significant potential as a rapid, sensitive, and specific assay for diagnosing infections caused by this organism. One of the remaining issues regarding the use of this technique is the extraction of DNA from clinical material prior to performing the PCR. It requires the use of DNA extraction method that can efficiently lyse mycobacterial cells and recover small amounts of DNA. The method of heating the sample in a boiling water bath to break...

The purpose of this study was to assess the level of fasting plasma glucose, zinc level, C-peptide level and HbA1c in type II diabetes mellitus (DM) population of Bangladesh and to correlate the values with several markers of zinc level. Since Zinc plays a clear role in the synthesis, storage and secretion of insulin as well as conformational integrity of insulin in hexameric form, the decreased Zn, which affects the ability of the islet cell to produce and secrete insulin, might then compound the problem, particularly in Type II diabetes.This study was conducted in Nutrition lab, Department...

During the past few years, interest in the development of novel drug delivery systems for existing drug molecules has been renewed. The development of a novel delivery system for existing drug molecules not only improves the drug's performance in terms of efficacy and safety but also improves patient compliance and overall therapeutic benefit to a significant extent. Transdermally delivered drugs avoid the risk and inconvenience of intravenous therapy, usually provide less chance of an overdose or underdose, allow easy termination, and permit both local and systemic treatment effects....