The present project was designed to study the prevalence of Vitamin D, calcium and phosphate in healthy females in Lahore. In this study 88 blood samples of healthy females were used to assay the concentration of Vitamin D, calcium and phosphate. ELISA was performed for the assessment of Vitamin D in the laboratory of LCWU. The calcium and phosphate were measured by using commercially available kits on chemistry analyzer. Statistically data was analyzed by using SPSS (version 13.0). The mean value of Vitamin D was 42.76 nmol 2.05. The mean of calcium was 8.78 mg/dl 0.06 and the mean of...