Newcastle disease (ND) is the principal killer of rural poultry in Asian and African countries. In order to prevent further spread of ND virus in outbreak areas, faster methods of diagnosis are required. Haemagglutination inhibition test and ELISA are the most widely used methods. However, these tests are either labor-intensive, costly or require two samples, collected at least two weeks apart, to differentiate between antibodies due to vaccination or active infection. PCR, which can be used in earlier days of outbreak and with higher sensitivity and specificity requires at least 90 minutes...