This Book describes the studies of field isolated Brucella melitensis, B.abortus in comparison with reference and vaccine strains. General bacteriological and serological examinations were used to isolate the organism from small ruminants (B. melitensis) and bovine species (B. abortus). omp31, omp2a and IS711 genes have been used as a target gene for PCR amplification to identify the field isolates and to differentiate between them. Cloning and sequencing of omp31 gene in this monograph showed 99% similarity to most Brucella omp31 genes. Further homology search for other omps of Brucella...