Modern livestock producer employs artificial insemination (AI) using cryopreserved semen. The objective of this study is to determine the viability of sperms before and after cryopreservation. Semen collected from Bos taurus (4 Jersey cross and 8 Jersey pure breeds) was diluted to 80x106 sperms/ml, packed in 0.25ml French plastic straws and cryopreserved in liquid nitrogen. Microbial load assessment was performed to determine the quality. The present study aims at estimating the percentage of damage on the cryopreserved sperm by assessing Motility, Plasma Membrane and Acrosomal Integrity....