This study aimed to isolate, purify and characterize L-asparaginase present in some animal tissues. The L-asparaginase (EC 3.5.1.1) produced by chicken livers was isolated and characterized. Different purification steps (including ammonium sulphate fractionation followed by separation on Sephadex G-100 gel filtration and Sephadex G-200 gel filtration) were applied to crude filtrate to obtain a pure enzyme preparation. The enzyme was purified 128.5 0.5 fold and showed a final specific activity of 158.11 5.0 U/mg with a 17.1 8.6 % yield. Sodium dodecyl sulphate polyacrylamide gel...