This Monograph describes the impact of a potent lipase producing soil bacterial strain Acinetobacter sp. The characterization of crude lipase from the optimized media showed that lipolytic activity was optimal at pH 8.5 and at 30 C. Lipase activity was stable over a wide pH range 4.0 - 9.5] and significant temperature stability up to 50 C at pH 8.5 was observed. Lipase was stable in commercially available detergents and it was found to be remarkably activated by various salts and also stable in 40%, 80% organic solvents and hydrogen peroxide. Partial purification using 40% ammonium sulphate...